Referenced in: none

Automatically associated channels: Kir1.1 , Kir4.2

Title: Differential gene expression profiles of normal human parotid and submandibular glands.

Authors: Q-F Sun, Q-H Sun, J Du, S Wang

Journal, date & volume: , 2008 Sep , 14, 500-9

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/18826381

Abstract
Parotid and submandibular glands have different properties including characteristics of the secreted saliva and tumor incidences. The differences in properties of parotid and submandibular glands are not clear from a genetic viewpoint.To study differential gene expression profiles between normal human parotid and submandibular glands.Three pairs of normal parotid and submandibular glands were obtained. RNA was extracted from these samples. After reverse transcription, the cDNA was in vitro-transcribed to produce biotin-labeled cRNA. The purified biotin-labeled cRNA samples were hybridized to microarray chips.Among the 54 675 tested transcripts, 47 transcripts were upregulated at least twofold in the parotid gland compared with the submandibular gland, including tumor-associated genes (pleiotrophin, WNT5A, ABCC1) and transport-associated genes (SLCO1A2, SLC13A5, KCNJ15). Ninety-eight transcripts were upregulated at least twofold in the submandibular gland compared with the parotid gland, including the chloride channel CFTR and mucin-associated genes that belong to the starch and sucrose metabolism pathway (GalNAc-T4, GalNAc-T7 and GalNAc-T13). Quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of nine differentially expressed genes confirmed the microarray results.This study revealed the different gene expression profiles of normal human parotid and submandibular glands, providing a genetic basis for their differing properties.