Referenced in: none

Automatically associated channels: Cav3.2

Title: [The effect of CACNA1H gene G773D mutation on calcium channel function]

Authors: Ju-li Wang, Chong-Yang Han, Yu-hong Jing, Yu-cai Chen, Nan Feng, Jian-jun Lu, Yue-Hua Zhang, Hong Pan, Hu-sheng Wu, Ke-ming Xu, Yu-wu Jiang, Jian-min Liang, Ling Wang, Xiao-Liang Wang, Yan Shen, Xi-ru Wu

Journal, date & volume: Zhonghua Yi Xue Yi Chuan Xue Za Zhi, 2006 Aug , 23, 369-73

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/16883519

Abstract
To study the effect of CACNA1H gene mutation G773D on calcium channel function.By the overlap extension PCR we introduced G773D mutation into a human Cav3.2acDNA for constructing the mutant. And then using whole cell clamp technique, we studied the alterations of channel behavior in transfected HEK-293 cells.There were no difference in kinetics of activation and inactivation of calcium channel between wild type and mutant. However comparing with the wild-type Cav3.2 channel, G773D mutant could increase the calcium current density significantly.CACNA1H gene G773D mutation is able to increase calcium current and neuronal excitability.