Referenced in: none

Automatically associated channels: Kv10.1

Title: P2X7-induced apoptosis decreases by aging in mice myeloblasts.

Authors: Edgar Julian Paredes-Gamero, Juliana Luporini Dreyfuss, Helena B Nader, Maria Etsuko Miyamoto Oshiro, Alice Teixeira Ferreira

Journal, date & volume: Exp. Gerontol., 2007 Apr , 42, 320-6

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/17188441

Abstract
In the current study, the ability of ATP to promote apoptosis in myeloblasts at different ages was investigated. We have observed that high concentration of extracellular ATP (>1mM), which activates P2X(7) receptor, produced cell shrinkage an increase in the number of events in the sub-G(0)/G(1) region of the cellular cycle and annexin-V/propidium iodide label, which characterizes the apoptotic cell death. In addition, BzATP produced apoptosis, but not ADP and UTP. Gr-1(+) cells express the P2X(7) receptor and oxidized ATP, a specific P2X(7) inhibitor, blocked the ATP-dependent apoptosis. ATP-dependent apoptosis is decreased by aging in myeloblasts of 12 and 22-month-old mice. Furthermore, P2X(7) expression decrease was observed in older mice, explaining apoptosis decrease. This decrease in apoptosis by aging may be related to some diseases in the myelocyte lineage.