Referenced in: none

Automatically associated channels: Kv10.1 , Kv11.1 , Kv8.2

Title: Resonance assignment and secondary structure prediction of the N-terminal domain of hERG (Kv11.1).

Authors: Frederick W Muskett, John S Mitcheson

Journal, date & volume: , 2010 Aug 14 , ,

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/20711762

Abstract
The hERG (human ether-à-go-go related gene) channel is a member of the eag voltage-gated K(+) channel family. In common with other members of this family, it has a subunit topology of six trans-membrane helices that tetramerise to form a functional ion-channel. In addition, hERG has an N-terminal PAS (Per, Arnt and Sim) domain and a C-terminal cyclic nucleotide binding domain (cNBD). Both these cytosolic domains are involved in regulation of the gating of the ion channel as demonstrated by inheritable mutations in these domains that result in either a loss, or a gain, in function. Here we report near complete backbone and side chain (15)N, (13)C and (1)H assignments for the N-terminal domain (residues 1-135) including the functionally critical first 26 residues. Comparison with the secondary structure of the crystal structure (residues 26-135) suggests that the solution and crystal structures are very similar except that the solution structure contains an additional helix between residues 12-23; a region of the protein important for channel gating.