Referenced in: none

Automatically associated channels: Slo1

Title: [Activation of cloned BKCa channels in Ang II-induced proliferation of HEK293 cells]

Authors: Jun Li, Chang-Lei Deng, Quan Li, Zhi-Bin Yu, Yao-ming Chang, Li Liu, Man-Jiang Xie

Journal, date & volume: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 2009 Jul , 25, 584-7

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/19737473

Abstract
To investigate the mediating and regulating role of BK(Ca) channels in Ang II-induced cell proliferation.Using Lipofectamine 2000, the pIRES-hSloalpha plasmid was transfected into HEK293 cells. The concentration-dependent cuve of Ang II-induced cell proliferation was tested by MTT colorimetry. The effect of IbTX, Ang II, Ang II + IbTX on the proliferating cell nuclear antigen (PCNA) expression and cell cycle of transfected HEK293-hSloalpha cells were detected by immunocytochemistry and flow cytometry, respectively.Ang II induced proliferation of transfected HEK293-hSloalpha cells in a concentration-dependent manner. PCNA expression of transfected HEK293-hSloalpha cells was enhanced by Ang II, and the percentage of S phase HEK293-hSloalpha cells was increased after Ang II treatment. However this effect was inhibited by IbTX, a selective BK(Ca) channel blocker.BK(Ca) channels play an potential role in mediating Ang II-induced proliferation of HEK293-hSloalpha cells.