Channelpedia

PubMed 19574640


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Kv1.3



Title: Crystallization and preliminary X-ray diffraction studies of the tetramerization domain derived from the human potassium channel Kv1.3.

Authors: Andreas Winklmeier, Michael Weyand, Christina Schreier, Hans Robert Kalbitzer, Werner Kremer

Journal, date & volume: Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun., 2009 Jul 1 , 65, 688-91

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/19574640


Abstract
The tetramerization domain (T1 domain) derived from the voltage-dependent potassium channel Kv1.3 of Homo sapiens was recombinantly expressed in Escherichia coli and purified. The crystals were first grown in an NMR tube in 150 mM potassium phosphate pH 6.5 in the absence of additional precipitants. The crystals showed I4 symmetry characteristic of the naturally occurring tetrameric assembly of the single subunits. A complete native data set was collected to 1.2 A resolution at 100 K using synchrotron radiation.