Referenced in: none

Automatically associated channels: Kv11.1

Title: Modulation of plasma membrane lipid profile and microdomains by H2O2 in Saccharomyces cerevisiae.

Authors: Nuno Pedroso, Ana C Matias, Luísa Cyrne, Fernando Antunes, Carlos Borges, Rui Malhó, Rodrigo F M de Almeida, Enrique Herrero, H Susana Marinho

Journal, date & volume: Free Radic. Biol. Med., 2009 Jan 15 , 46, 289-98

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/19027845

Abstract
In Saccharomyces cerevisiae, the rate of hydrogen peroxide (H(2)O(2)) diffusion through the plasma membrane decreases during adaptation to H(2)O(2) by a still unknown mechanism. Here, adaptation to H(2)O(2) was observed to modulate rapidly the expression of genes coding for enzymes involved in ergosterol and lipid metabolism. Adaptation to H(2)O(2) also alters plasma membrane lipid composition. The main changes were the following: (a) there was a decrease in oleic acid (30%) and in the ratio between unsaturated and saturated long-chain fatty acids; (b) the phosphatidylcholine:phosphatidylethanolamine ratio increased threefold; (c) sterol levels were unaltered but there was an increased heterogeneity of sterol-rich microdomains and increased ordered domains; (d) the levels of the sterol precursor squalene increased twofold, in agreement with ERG1 gene down-regulation; and (e) C26:0 became the major very long chain fatty acid owing to an 80% decrease in 2-hydroxy-C26:0 levels and a 50% decrease in C20:0 levels, probably related to the down-regulation of fatty acid elongation (FAS1, FEN1, SUR4) and ceramide synthase (LIP1, LAC1) genes. Therefore, H(2)O(2) leads to a reorganization of the plasma membrane microdomains, which may explain the lower permeability to H(2)O(2), and emerges as an important regulator of lipid metabolism and plasma membrane lipid composition.