Referenced in: none

Automatically associated channels: Kv10.1

Title: [Transport of Rb+ via activated sodium channels of clone N 18 phi 1 neuroblastoma cells]

Authors: N M Gulaia, V K Lishko, G L Volkov, N N Govseeva

Journal, date & volume: Ukr. Biokhim. Zh., 1983 Nov-Dec , 55, 657-61

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/6318414

Abstract
A study was made of the Rb+ transport via activated sodium channels of clone N 18 phi 1 neuroblastoma cells cultured in the Eagle medium with 10% bovine serum. The time of population doubling was about 10 h. The cell differentiation was induced by adding bromdeoxyuridine in a concentration of 1-4 10(-5) M. The cells contained 172 +/- 12 and 340 +/- 35 micrograms of protein per 10(6) cells at the logarithmic growth phase and in differentiated state, respectively. It is shown that veratrin produced a 1.3-fold increase in the rate of 86Rb+ removal from undifferentiated cells and 2.5-fold increase in that from differentiated cells. Tetrodotoxin removed completely the effect of veratrin. A conclusion is made on the presence of a new clone of fast sodium channels in cell membranes.