Referenced in: none

Automatically associated channels: Kv10.1 , Slo1

Title: Sulfhydryl groups are involved in H+ translocation via the uncoupling protein of brown adipose tissue mitochondria.

Authors: P Jezek

Journal, date & volume: FEBS Lett., 1987 Jan 19 , 211, 89-93

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/3026844

Abstract
Mersalyl inhibits H+ transport via the uncoupling protein (UP) in brown adipose tissue (BAT) mitochondria estimated as swelling in potassium acetate (Ki 67 microM) or as valinomycin-induced H+ extrusion in K2SO4 (Ki 55 microM) and KCl. The swelling in KCl is depressed only slightly. Some other SH-reagents (p-hydroxymercuribenzoate, 5,5'-dithiobis(2-nitrobenzoate) and thiolyte DB), but not hydrophobic reagents (N-ethylmaleimide and eosin-5-maleimide), exhibit analogous inhibition. Thus an essential SH-group localized at the water-accessible cytosolic surface of UP was found to be involved in H+ transport via UP but not in Cl- transport.