Referenced in: none

Automatically associated channels: Kv10.1

Title: Intensification of rheological destruction of rat fibrosarcoma KMT 17 cells by elimination of divalent cations.

Authors: T Koyama, T Araiso, M Ishikawa, F Okada, H Kobayashi

Journal, date & volume: , 1987 , 24, 775-82

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/3139103

Abstract
In an effort to find a method to intensify the rheological destruction of tumor cells, rat fibrosarcoma KMT 17 cells in ascitic form were exposed to rheological stresses in test solutions; modified Eagle medium with EDTA, and phosphate buffer solution without divalent cations or with verapamil. KMT 17 cells were exposed to a uniform shear stress produced by the rotation of a rotating cone plate viscometer for 1 to 2 hours and to the strong deformation by the passage through Nuclepores of 10, 8 and 5 micrometer. KMT 17 cells suspended in the test solutions were more effectively destroyed by the stresses than those suspended in normal solutions containing divalent cations without any other calcium-suppressing agents. These results suggest that the elimination of divalent cations and/or the block of calcium ion channels of cell membranes intensify the rheological destruction of tumor cells migrating in the circulatory system.