Referenced in: none

Automatically associated channels: Kv10.1

Title: 1,4-Dihydropyridine receptor associated with Ca2+ channels in human embryonic fibroblasts.

Authors: S M Dudkin, S N Gnedoj, N N Chernyuk, N M Soldatov

Journal, date & volume: FEBS Lett., 1988 Jun 20 , 233, 352-4

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/2454846

Abstract
By using the radioactively labeled 1,4-dihydropyridine (DHP) probe, [3H]PMD, we have demonstrated that cultured human embryonic fibroblasts grown at a low density in Eagle's medium supplemented with serum contain a single class of non-interacting DHP binding sites (Bmax, 1.2 +/- 0.3 pmol/10(6) cells; Kd, 3.9 nM). After inhibition of the DHP receptor biosynthesis by cycloheximide, the number of [3H]PMD binding sites is reduced with a half-time of 12 h, which implies a turnover rate of 30,000 +/- 7500 receptors/h per cell. With progression to confluency, the Bmax value decreased up to 0.28 +/- 0.08 pmol/10(6) cells without significant change in Kd value. When cells were grown at a low density in serum-free conditions, the number of [3H]PMD binding sites gradually increased 1.9-fold within 3 days. Addition of serum reversed this effect with the same time course. These results imply that the DHP-sensitive Ca2+ channels are involved in the control of the proliferation of human embryonic fibroblasts.