Channelpedia

PubMed 2539482


Referenced in: none

Automatically associated channels: Kv2.1



Title: Crystallization of porin using short chain phospholipids.

Authors: J L Eiselé, J P Rosenbusch

Journal, date & volume: J. Mol. Biol., 1989 Mar 5 , 206, 209-12

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/2539482


Abstract
A protein from outer membranes of Escherichia coli K-12, porin OmpF was crystallized either with short acyl chain phospolipids or with various detergents as amphiphiles. In dihexanoyl phosphatidylcholine, crystals greater than 0.3 mm in all dimensions were obtained that diffract X-rays beyond 3.5 A. Crystal morphology and unit cell dimensions were indistinguishable from those of detergent-grown crystals, indicating that the role of the phospholipids used is similar to those of many conventional detergents.