Referenced in: none

Automatically associated channels: Kir2.3

Title: Fabrication of key components of a receptor-based biosensor.

Authors: F S Ligler, T L Fare, K D Seib, J W Smuda, A Singh, P Ahl, M E Ayers, A Dalziel, P Yager

Journal, date & volume: , 1988 Oct , 22, 247-56

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/2846991

Abstract
A three-pronged approach was taken to the development of receptor-based bisensors. First, asymmetric bilayer membranes were developed with one monolayer adaptable to the particular receptor of interest and the other monolayer polymerized to enhance membrane stability. Second, alamethicin and calcium channel complexes were introduced into the stabilized membrane and tested for ion-channel function. Third, a porous support for the receptor-containing membrane was fabricated, which is compatible with silicon technology. Preliminary devices incorporating these components were constructed.