Referenced in: none

Automatically associated channels: Kv2.1

Title: Non-selective cation and dysfunctional chloride channels in the apical membrane of nasal epithelial cells cultured from cystic fibrosis patients.

Authors: M Jorissen, J Vereecke, E Carmeliet, H Van den Berghe, J J Cassiman

Journal, date & volume: Biochim. Biophys. Acta, 1990 Nov 14 , 1096, 52-9

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/2176548

Abstract
Chloride channels and non-selective cation channels in the apical membranes of cultured nasal epithelial cells from three cystic fibrosis patients were investigated with the patch-clamp technique. Outwardly rectifying chloride channels were found in 31% of the inside-out patches, but activity of this channel was never observed in cell-attached patches, even after stimulation with adrenaline. In 30% of the patches with chloride channels, activation occurred immediately after excision. Most of the channels, however, activated only after a membrane depolarization of +40 to +120 mV. Once activated, the chloride channels were indistinguishable from those in nasal epithelial cells of control patients. Amiloride-insensitive, calcium- and voltage-dependent, non-selective cation channels were present in 11% of the cell-attached and 43% of the cell-free patches and could not be distinguished from those in controls. The cystic fibrosis chloride channel defect is conserved in cultured nasal epithelial cells, while a non-selective cation channel is apparently not affected.