Referenced in: none

Automatically associated channels: Kv10.1 , Kv11.1

Title: Silencing of herg gene by shRNA inhibits SH-SY5Y cell growth in vitro and in vivo.

Authors: Jie Zhao, Xiao-Li Wei, Yong-Sheng Jia, Jian-quan Zheng

Journal, date & volume: Eur. J. Pharmacol., 2008 Jan 28 , 579, 50-7

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/17976575

Abstract
Overexpression of human ether-à-go-go (eag) related gene (herg) contributes to the progression and metastasis of a variety of tumors of different histogenesis, which implies that the herg gene could provide a promising target on tumor therapy. In the present study, plasmid-mediated expression of shRNA-herg1 and shRNA-herg1/1b was employed to silence the herg gene expression in human neuroblastoma SH-SY5Y cell lines. The inhibition of the target gene expression was confirmed by RT-PCR and Western blot. It was found that shRNA-herg1 or shRNA-herg1/1b depressed the cellular growth rate, inhibited cell viability and reduced colony formation of SH-SY5Y cells. The flow cytometry assay revealed that SH-SY5Y cells were retarded in G0-G1 after herg1 or herg1/1b gene was silenced by shRNA-herg1 or shRNA-herg1/1b. In vivo, intra-tumor injection of shRNA-herg1/1b inhibited the growth of SH-SY5Y tumors inoculated subcutaneously in nude mice. The result suggested that the herg played an important role in regulating the growth and proliferation of SH-SY5Y cells. The block of the HERG channel might be a potential therapeutic strategy for neuroblastoma and some other tumors with overexpression of the herg gene.