Title: Contactin associates with Na+ channels and increases their functional expression.

Authors: K Kazarinova-Noyes, J D Malhotra, D P McEwen, L N Mattei, E O Berglund, B Ranscht, S R Levinson, M Schachner, P Shrager, L L Isom, Z C Xiao

Journal, date & volume: J. Neurosci., 2001 Oct 1 , 21, 7517-25

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/11567041

Abstract
Contactin (also known as F3, F11) is a surface glycoprotein that has significant homology with the beta2 subunit of voltage-gated Na(+) channels. Contactin and Na(+) channels can be reciprocally coimmunoprecipitated from brain homogenates, indicating association within a complex. Cells cotransfected with Na(+) channel Na(v)1.2alpha and beta1 subunits and contactin have threefold to fourfold higher peak Na(+) currents than cells with Na(v)1.2alpha alone, Na(v)1.2/beta1, Na(v)1.2/contactin, or Na(v)1.2/beta1/beta2. These cells also have a correspondingly higher saxitoxin binding, suggesting an increased Na(+) channel surface membrane density. Coimmunoprecipitation of different subunits from cell lines shows that contactin interacts specifically with the beta1 subunit. In the PNS, immunocytochemical studies show a transient colocalization of contactin and Na(+) channels at new nodes of Ranvier forming during remyelination. In the CNS, there is a particularly high level of colocalization of Na(+) channels and contactin at nodes both during development and in the adult. Contactin may thus significantly influence the functional expression and distribution of Na(+) channels in neurons.