Title: Deglycosylation altered the gating properties of rNav1.3: glycosylation/deglycosylation homeostasis probably complicates the functional regulation of voltage-gated sodium channel.

Authors: Qing Xu, Hui-Wen Cheng, Hui-Qiong He, Zhi-Rui Liu, Ming He, Hong-Tian Yang, Zhi-Lei Zhou, Yong-Hua Ji

Journal, date & volume: , 2008 Oct , 24, 283-7

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/18839021

Abstract
To examine the effect of deglycosylation on gating properties of rNav1.3.rNav1.3 was expressed in Xenopus oocyte, with glycosylation inhibition by using tunicamycin. Two-electrode voltage clamp was employed to record the whole-cell sodium current and data were analyzed by Origin software. Those of glycosylated rNav1.3 were kept as control.Compared with glycosylated ones, the steady-state activation curve of deglycosylated rNav1.3 was positively shifted by about 10 mV, while inactivation curve was negatively shifted by about 8 mV.Glycosylation altered the gating properties of rNav1.3 and contributed to the functional diversity.观察糖基化对rNav1.3电压门控性质的影响。将rNav1.3在爪蟾卵母细胞中表达, 衣霉素抑制其糖基化, 双电极电压钳记录全细胞电流, Origin软件分析处理数据。 对照组为未去糖基化的rNav1.3。与糖基化的rNav1.3相比, 去糖基化的rNav1.3稳态激活曲线向去极化方向偏移约10 mV, 稳态失活曲线向超极化方向偏移约8 mV。糖基化修饰改变rNav1.3的电压门控性质, 异化其生理功能。