Referenced in: none

Automatically associated channels: ClC2 , ClC4

Title: ClC-2 activation modulates regulatory volume decrease.

Authors: H Xiong, C Li, E Garami, Y Wang, M Ramjeesingh, K Galley, C E Bear

Journal, date & volume: J. Membr. Biol., 1999 Feb 1 , 167, 215-21

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/9929373

Abstract
ClC-2 belongs to a large family of chloride channels and its expression in certain cell types is associated with the appearance of swelling-activated chloride (Cl-) currents. In the present report, we examined the hypothesis that ClC-2 plays a role in regulatory volume decrease by expressing ClC-2 in Sf9 cells using the baculovirus system. First, we showed that ClC-2 protein expression is associated with appearance of a Cl- conductance which is activated by hypo-osmotic shock and can be distinguished from swelling-activated chloride currents endogenous to Sf9 cells on the basis of its pharmacology and specific inhibition by an anti-ClC-2 antibody. Second, we show that the rate of regulatory volume decrease is significantly enhanced in Sf9 cells expressing ClC-2 protein. Hence, our data support the hypothesis that ClC-2 is capable of mediating regulatory volume decrease.