Referenced in: none

Automatically associated channels: SK1 , TRP , TRPV , TRPV4

Title: The passive and active contractile properties of the neurogenic, underactive bladder.

Authors: John S Young, Louise Johnston, Camille Soubrane, Karen D McCloskey, Gordon McMurray, Rachel Eccles, Christopher H Fry

Journal, date & volume: BJU Int., 2013 Feb , 111, 355-61

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/22712666

Abstract
To characterize passive and active changes in detrusor activity in a highly compliant bladder.Bladders from adult female Sprague-Dawley rats were used 5 weeks after lower thoracic (T8) spinal cord transection or a sham-operation. Passive wall properties were assessed by pressure-volume relationships from whole bladders and the tensile response of bladder strips after a rapid (<0.5 s) stretch. Active properties were assessed from the frequency and amplitude of spontaneous contractions of bladder strips, and their response to the inotropic TRPV4 agonist GSK1016790A.Passive bladder wall stiffness of SCT bladders was significantly reduced compared to that of the sham-operated control group (N = 6 and 8, respectively) and SCT bladder strips relaxed more quickly than those from sham-operated rats. The frequency of spontaneous contractions was reduced in SCT rats, and their amplitude, expressed as a ratio of bladder wall stiffness, was greater than in sham-operated rats. GSK1016790A (0.1 μM) significantly increased amplitude in strips from both sham-operated and SCT groups.There is no evidence of contractile failure in a highly-compliant bladder. The observations of reduced passive bladder wall stiffness and an enhanced rate of stress relaxation lead to the conclusion that increased compliance is marked by altered matrix properties that dissipate muscle force, thereby generating low pressures. Contractile agonists may be effective for improving bladder function in detrusor underactivity.