Referenced in: none

Automatically associated channels: Kv3.4

Title: The increase of voltage-gated potassium channel Kv3.4 mRNA expression in oral squamous cell carcinoma.

Authors: Kuo-Wei Chang, Ta-Chun Yuan, Kung-Ping Fang, Feng-Shuo Yang, Chung-Ji Liu, Che-Shoa Chang, Shu-Chun Lin

Journal, date & volume: J. Oral Pathol. Med., 2003 Nov , 32, 606-11

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/14632936

Abstract
Potassium channels have been reported to be involved in the proliferation of many types of cells, including tumor cells. The overexpression of the K+ channel and related channel activity are involved in the neoplastic process.We examined the expression of an A-type voltage-gated K+ channel, Kv3.4, in oral squamous cell carcinoma (OSCC) and esophageal squamous cell carcinoma (ESCC) compared with non-cancerous matched tissue (NCMT) using RT-PCR analysis. In addition, administration of an A-type K+ channel blocker, 4-aminopyridine (4-AP), and an antisense oligodeoxynucleotide (ODN) directed specifically against Kv3.4 were performed to identify the involvement of Kv3.4 in the growth of OSCC cells.A significantly increase in the frequency of Kv3.4 mRNA expression was identified in OSCC (64%) compared to corresponding NCMT (29%) (P = 0.05). The increase of Kv3.4 mRNA expression was also eminent in ESCC. Growth of OSCC cells was significantly inhibited by 4-AP in a dose-dependent manner at different time point of treatment. In OECM-1 OSCC cells, a significant growth inhibition was noted in antisense ODN-treated cells compared to control cells.We provide novel evidences of the increase of Kv3.4 mRNA expression in OSCC. The abrogation of Kv3.4 inhibits the growth of OSCC cells.