Referenced in: none

Automatically associated channels: Kv1.1 , Kv1.6 , Kv2.1 , Nav1.5

Title: Involvement of Kv1.1 and Nav1.5 in proliferation of gastric epithelial cells.

Authors: William Ka Kei Wu, Gui Rong Li, Helen Pui Shan Wong, Marco King Cheung Hui, Emily Kin Ki Tai, Emily Kai Yee Lam, Vivian Yvonne Shin, Yi Ni Ye, Peng Li, Ying Hua Yang, Jiing Chyuan Luo, Chi Hin Cho

Journal, date & volume: J. Cell. Physiol., 2006 May , 207, 437-44

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/16331678

Abstract
In the present study, patch clamp experiments demonstrated the expression of multiple ionic currents, including a Ba2+-sensitive inward rectifier K+ current (IKir), a 4-aminopyridine- (4-AP) sensitive delayed rectifier K+ current (IKDR), and a nifedipine-sensitive, tetrodotoxin-resistant inward Na+ current (INa.TTXR) in the non-transformed rat gastric epithelial cell line RGM-1. RT-PCR revealed molecular identities of mRNAs for the functional ionic currents, including Kir1.2 for IKir, Kv1.1, Kv1.6, and Kv2.1 for IKDR, and Nav1.5 for INa.TTXR. Pharmacologic blockade of Kv and Nav, but not Kir, suppressed RGM-1 cell proliferation. To further elucidate which subtypes of the ion channels were involved in cell proliferation, RNA interference was employed to knockdown specific gene expression. Downregulation of Kv1.1 or Nav1.5 by RNA interference suppressed RGM-1 cell proliferation. To conclude, our study is the first to delineate the expression of ion channels and their functions as growth modulators in gastric epithelial cells.