Referenced in: none

Automatically associated channels: Kv10.1 , Kv3.1

Title: K+ channel KV3.1 associates with OSP/claudin-11 and regulates oligodendrocyte development.

Authors: Seema Tiwari-Woodruff, Luis Beltran-Parrazal, Andrew Charles, Thomas Keck, Trung Vu, Jeff Bronstein

Journal, date & volume: Am. J. Physiol., Cell Physiol., 2006 Oct , 291, C687-98

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/16624990

Abstract
K(+) channels are differentially expressed throughout oligodendrocyte (Olg) development. K(V)1 family voltage-sensitive K(+) channels have been implicated in proliferation and migration of Olg progenitor cell (OPC) stage, and inward rectifier K+ channels (K(IR))4.1 are required for OPC differentiation to myelin-forming Olg. In this report we have identified a Shaw family K(+) channel, K(V)3.1, that is involved in proliferation and migration of OPC and axon myelination. Application of anti-K(V)3.1 antibody or knockout of Kv3.1 gene decreased the sustained K(+) current component of OPC by 50% and 75%, respectively. In functional assays block of K(V)3.1-specific currents or knockout of Kv3.1 gene inhibited proliferation and migration of OPC. Adult Kv3.1 gene-knockout mice had decreased diameter of axons and decreased thickness of myelin in optic nerves compared with age-matched wild-type littermates. Additionally, K(V)3.1 was identified as an associated protein of Olg-specific protein (OSP)/claudin-11 via yeast two-hybrid analysis, which was confirmed by coimmunoprecipitation and coimmunohistochemistry. In summary, the K(V)3.1 K(+) current accounts for a significant component of the total K(+) current in cells of the Olg lineage and, in association with OSP/claudin-11, plays a significant role in OPC proliferation and migration and myelination of axons.