Referenced in: none

Automatically associated channels: Kv1.3

Title: Solid-state NMR spectroscopy applied to a chimeric potassium channel in lipid bilayers.

Authors: Robert Schneider, Christian Ader, Adam Lange, Karin Giller, Sönke Hornig, Olaf Pongs, Stefan Becker, Marc Baldus

Journal, date & volume: J. Am. Chem. Soc., 2008 Jun 11 , 130, 7427-35

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/18479093

Abstract
We show that solid-state NMR can be used to investigate the structure and dynamics of a chimeric potassium channel, KcsA-Kv1.3, in lipid bilayers. Sequential resonance assignments were obtained using a combination of (15)N- (13)C and (13)C- (13)C correlation experiments conducted on fully labeled and reverse-labeled as well as C-terminally truncated samples. Comparison of our results with those from X-ray crystallography and solution-state NMR in micelles on the closely related KcsA K (+) channel provides insight into the mechanism of ion channel selectivity and underlines the important role of the lipid environment for membrane protein structure and function.