Referenced in: none

Automatically associated channels: ClC1 , ClC4

Title: Low single channel conductance of the major skeletal muscle chloride channel, ClC-1.

Authors: M Pusch, K Steinmeyer, T J Jentsch

Journal, date & volume: Biophys. J., 1994 Jan , 66, 149-52

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/8130334

Abstract
We expressed the skeletal muscle chloride channel, ClC-1, in HEK293 cells and investigated it with the patch-clamp technique. Macroscopic properties are similar to those obtained after expression in Xenopus oocytes, except that faster gating kinetics are observed in mammalian cells. Nonstationary noise analysis revealed that both rat and human ClC-1 have a low single channel conductance of about 1 pS. This finding may explain the lack of single-channel data for chloride channels from skeletal muscle despite its high macroscopic chloride conductance.