Referenced in: none

Automatically associated channels: Kv10.1

Title: Selective inhibition of the mitochondrial permeability transition pore at the oxidation-reduction sensitive dithiol by monobromobimane.

Authors: P Costantini, B V Chernyak, V Petronilli, P Bernardi

Journal, date & volume: FEBS Lett., 1995 Apr 3 , 362, 239-42

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/7536690

Abstract
In this paper we introduce monobromobimane, a thiol reagent, as a selective blocker of the recently identified dithiol whose oxidation-reduction status modifies voltage sensing by the mitochondrial permeability transition pore, a cyclosporin A-sensitive channel. Monobromobimane does not inhibit the phosphate carrier, nor does it interfere with Ca2+ transport, energy coupling or ATP production and transport. We show that monobromobimane selectively prevents the shift in pore gating potential caused by some dithiol oxidants or crosslinkers but not by increasing [Ca2+], allowing a clear distinction of the pore agonists which act at this site.