Referenced in: none

Automatically associated channels: ClC3 , ClC4 , ClC5

Title: Cloning and functional expression of rat CLC-5, a chloride channel related to kidney disease.

Authors: K Steinmeyer, B Schwappach, M Bens, A Vandewalle, T J Jentsch

Journal, date & volume: J. Biol. Chem., 1995 Dec 29 , 270, 31172-7

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/8537381

Abstract
We have cloned a novel member of the CLC chloride channel family from rat brain, rCLC-5. The cDNA predicts a 83-kDa protein belonging to the branch including CLC-3 and CLC-4, with which it shares approximately 80% identity. Expression of rCLC-5 in Xenopus oocytes elicits novel anion currents. They are strongly outwardly rectifying and have a conductivity sequence of NO3- > Cl- > Br- > I- >> glutamate-. Although CLC-5 has consensus sites for phosphorylation by protein kinase A, raising the intracellular cAMP concentration had no effect on these currents. Currents were also unchanged when rCLC-5 was coexpressed with rCLC-3 and rCLC-4, either singly or in combination. rCLC-5 is expressed predominantly in kidney and also in brain, lung, and liver. Along the nephron, rCLC-5 message is detectable in all tubule segments investigated, but expression in the glomerulus and the S2 segment of the proximal tubule is low.