Referenced in: none

Automatically associated channels: Kir2.1 , Kv3.4

Title: The use of the rat LAP LCR and promoter for the high-level constitutive expression of K+ channel cDNAs in a rat liver cell line.

Authors: P A Shelton, C Dart

Journal, date & volume: FEBS Lett., 1996 Aug 12 , 391, 282-6

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/8764990

Abstract
Locus control regions (LCRs) are cis-acting elements that confer position-independent and copy-number-dependent expression upon associated genes in transgenic mice. Here we show the second example of the use of an LCR (the rat LAP LCR) in a stable expression vector system, used here in conjunction with the rat liver (NRLM) cell line. Non-transfected NRLM cells are electrically silent and highly suitable for patch clamp electrophysiology. We report reliable constitutive expression from two different K+ channel cDNAs; the voltage-gated rat clone Kv3.4 and the inward rectifier mouse clone Kir2.1. We further show that constitutive expression levels are stable for at least 8 weeks from initial recording.