Referenced in: none

Automatically associated channels: Kir2.1 , Kir2.2 , Kir3.1 , Kir3.2

Title: Activation of inwardly rectifying K+ channels by GABA-B receptors expressed in Xenopus oocytes.

Authors: Y Uezono, M Akihara, M Kaibara, C Kawano, I Shibuya, Y Ueda, N Yanagihara, Y Toyohira, H Yamashita, K Taniyama, F Izumi

Journal, date & volume: Neuroreport, 1998 Mar 9 , 9, 583-7

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/9559920

Abstract
In Xenopus oocytes coinjected with poly(A)+ RNA derived from the rat cerebellum and cRNAs for the cloned G protein-gated inwardly rectifying K+ channel (GIRK), GIRK1 and GIRK2, the GABA-B agonist baclofen elicited inwardly rectifying K+ currents. The inward K+ currents elicited by baclofen were inhibited by the selective GABA-B antagonists 2-OH saclofen and CGP 35348, and by the GIRK inhibitor Ba2+. In contrast, baclofen caused no currents in oocytes injected with the cerebellar poly(A)+ RNA alone, the poly(A)+ RNA and cRNA for GIRK1 or GIRK2, or only cRNAs for GIRK1 and GIRK2. These findings indicate that GABA-B receptors in the rat cerebellum were functionally expressed in Xenopus oocytes and activated the cloned GIRKs composed of GIRK1 and GIRK2 as heteromultimers.