Channelpedia

PubMed 19595989


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Kv1.3



Title: Protein dynamics detected in a membrane-embedded potassium channel using two-dimensional solid-state NMR spectroscopy.

Authors: Ader, Pongs, Becker, Baldus

Journal, date & volume: Biochim. Biophys. Acta, 2009 Jul 10 , ,

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/19595989


Abstract
We report longitudinal (15)N relaxation rates derived from two-dimensional ((15)N, (13)C) chemical shift correlation experiments obtained under magic angle spinning for the potassium channel KcsA-Kv1.3 reconstituted in multilamellar vesicles. Thus, we demonstrate that solid-state NMR can be used to probe residue-specific backbone dynamics in a membrane-embedded protein. Enhanced backbone mobility was detected for two glycine residues within the selectivity filter that are highly conserved in potassium channels and that are of core relevance to the filter structure and ion selectivity.