Referenced in: none

Automatically associated channels: Kir2.1 , Kir3.1

Title: Cloning and characterization of Kir3.1 (GIRK1) C-terminal alternative splice variants.

Authors: C S Nelson, J L Marino, C N Allen

Journal, date & volume: Brain Res. Mol. Brain Res., 1997 Jun , 46, 185-96

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/9191093

Abstract
Southern blot analysis of RT-PCR products from brain and heart revealed multiple products for a C-terminal region of Kir3.1. Sequencing yielded clones for wild-type Kir3.1 and three Kir3.1 C-terminal alternative splice variants, including a unique alternative exon. Two of these variants encoded truncated Kir3.1 molecules. Tissue distribution and electrophysiological characterization of a single truncated variant, Kir3.1(00) were then examined. Kir3.1 channels are gated by G-protein beta gamma-subunits binding to the C-terminal domain, thus, the truncation of Kir3.1(00) removes a major functional domain. When incorporated into heteromeric channels with other family members (Kir3.1, 3.2 or 3.4) several functional changes were observed: (1) Kir3.1(00) changes G-protein activation of Kir3 channels; (2) Kir3.1(00) is restricted in its ability to assemble with other channel subunits as heteromers; and (3) incorporation of Kir3.1(00) into heteromeric channel complexes alters the kinetics of channel re-activation.