Referenced in: none

Automatically associated channels: Kir2.3

Title: Transient inward current is conducted through two types of channels in cardiac Purkinje fibres.

Authors: X Han, G R Ferrier

Journal, date & volume: J. Mol. Cell. Cardiol., 1996 Oct , 28, 2069-84

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/8930803

Abstract
Two-microelectrode voltage-clamp technique was applied and a number of experimental manoeuvres were used to determine the charge-carrying systems of the arrhythmogenic transient inward current (TI) in rabbit cardiac Purkinje fibres. Increasing [Ca2+]o to 30 mmol/l (in the presence of [Na+]o) induced TI with a clear-cut reversal potential around -23 mV. This observation suggests that the TI is conducted through an ionic channel. Nickel chloride (2.5 mmol/l) which blocks Na+/Ca2+ exchange current greatly decreased peak inward TI (57 +/- 3%) but significantly increased peak outward TI (86.4 +/- 9.6%), which suggests that Na+ Ca2+ exchange is not the charge-carrying system for TI. In experiments in which TI was induced when [Na+]o was replaced by either N-methyl-D-glucamine, choline, or sucrose (to eliminate Na+ Ca2+ exchange), Ni2+ still decreased inward TI and increased outward TI. There was no significant difference between the effects of Ni2+ in the presence and absence of [Na+]o. The effects of Ni2+ in the absence of [Na+]o confirm that Ni(2+)-induced attenuation of inward TI is not mediated by the Na+ Ca2+ exchange, but rather through an inhibition of TI channels. Acute exposure to Mn2+ (5 mmol/l) almost abolished inward TI at a time when outward TI just showed a slight decrease. A third divalent cation, Cd2+ (0.25-1.0 mmol/l), strongly suppressed both inward and outward TI at the same time. The opposite effects of Ni2+ on inward v outward TI and the preferential inhibition of inward TI by Mn2- suggest involvement of multiple ionic channels in conducting TI. 4.4'-diisothiocyanatostilbene-2.2' disulfonic acid (DIDS, 10 mumol/l) and 4-acetamido-4'-isothicyanatostilbene-2.2' disulfonic acid (SITS. 0.2 mmol/l), which block CI- conductance in cardiac tissues, dramatically suppressed outward TI (80 +/- 9%) but to a lesser extent inward TI (20 +/- 4%). Our results suggest that, in cardiac Purkinje fibres, high [Ca2+]o induced TI is conducted mainly through TI channels which fall into two different populations: cationic and anionic.