Referenced in: none

Automatically associated channels: ClC4

Title: The yeast CLC chloride channel functions in cation homeostasis.

Authors: R A Gaxiola, D S Yuan, R D Klausner, G R Fink

Journal, date & volume: Proc. Natl. Acad. Sci. U.S.A., 1998 Mar 31 , 95, 4046-50

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/9520490

Abstract
A defect in the yeast GEF1 gene, a CLC chloride channel homolog leads to an iron requirement and cation sensitivity. The iron requirement is due to a failure to load Cu2+ onto a component of the iron uptake system, Fet3. This process, which requires both Gef1 and the Menkes disease Cu2+-ATPase yeast homolog Ccc2, occurs in late- or post-Golgi vesicles, where Gef1 and Ccc2 are localized. The defects of gef1 mutants can be suppressed by the introduction of Torpedo marmorata CLC-0 or Arabidopsis thaliana CLC-c and -d chloride channel genes. The functions of Gef1 in cation homeostasis provide clues to the understanding of diseases caused by chloride channel mutations in humans and cation toxicity in plants.