Channelpedia

PubMed 10591065


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Kir2.1



Title: The possible role of a disulphide bond in forming functional Kir2.1 potassium channels.

Authors: M L Leyland, C Dart, P J Spencer, M J Sutcliffe, P R Stanfield

Journal, date & volume: Pflugers Arch., 1999 Nov , 438, 778-81

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/10591065


Abstract
The role of two cysteine residues--Cys122 and Cys154--in the structure of the strong inward rectifier K+ channel, Kir2.1, has been investigated using site-directed mutagenesis and electrophysiology. Such cysteine residues are conserved across the inward rectifier family and may be expected to form a crucial disulphide bond. Our experiments show that when the cysteines are absent, the protein is expressed, but the channels are not functional, suggesting that the disulphide bond is essential for correct channel assembly. However, reducing agents applied extracellularly have little effect on current amplitude in wild-type, so that, once the channel is assembled correctly in the membrane, the disulphide bonds are no longer essential for function. Molecular modelling suggests that a disulphide bond is formed--this may be either an intra- or an inter-subunit.