Channelpedia

PubMed 10617917


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Kir2.1



Title: Kir2.1 Potassium channels and corneal epithelia.

Authors: J L Rae, A R Shepard

Journal, date & volume: Curr. Eye Res., 2000 Feb , 20, 144-52

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/10617917


Abstract
To determine the existence of inward rectifier (Kir2.1) sequences in mRNA from corneal epithelium and endothelium.cDNA library construction, cloning, PCR, patch clamp. RESULTS. Both the corneal epithelium and endothelium contain mRNA for Kir2.1 inwardly rectifying potassium channels. When the cDNA is transfected into Chinese hamster ovary cells, the channel currents match those expected from Kir2.1 inward rectifiers.The mRNA for Kir2.1 potassium channels exists in corneal epithelium and endothelium. Therefore, Kir2.1 inwardly rectifying potassium channels probably make some contributions to the resting voltages of cornea epithelium and endothelium. Previous data, however, suggest that they are probably not the dominant contributors in these preparations.