PubMed 10101018

Referenced in Channelpedia wiki pages of: none

Automatically associated channels: ClvC2 , ClvC4 , Kir6.2

Title: Keratinocyte growth factor stimulates CLC-2 expression in primary fetal rat distal lung epithelial cells.

Authors: C J Blaisdell, J P Pellettieri, C E Loughlin, S Chu, P L Zeitlin

Journal, date & volume: Am. J. Respir. Cell Mol. Biol., 1999 Apr , 20, 842-7

PubMed link:

Keratinocyte growth factor (KGF) is mitogenic for epithelial cells and induces cystic dilation of fetal lung explants through cystic fibrosis transmembrane conductance regulator-independent chloride channels. One candidate fetal lung chloride channel that is highly expressed on the apical surface of the respiratory epithelium and markedly downregulated after birth is CLC-2. We hypothesized that KGF regulates CLC-2 expression in the fetal lung. Primary fetal rat distal lung epithelial cell monolayers were grown in medium containing 10 ng/ml KGF for 48 h. CLC-2 protein was increased by Western blot analysis of whole-cell lysates in KGF-treated cultures. Similarly, KGF stimulated CLC-2 messenger RNA (mRNA) by Northern blot analysis. This enhanced expression was dose-dependent and maximal at 48 h with 10 ng/ml KGF. Promoter-reporter gene experiments demonstrated that KGF did not stimulate gene transcription. By inhibition of new mRNA synthesis with actinomycin D, evidence was obtained that KGF stabilizes CLC-2 mRNA. We speculate that KGF may positively influence pulmonary chloride and fluid secretion by a secondary pathway affecting CLC-2 degradation.