Referenced in: none

Automatically associated channels: ClC4 , ClC5

Title: Cloning, cellular distribution and functional expression of small intestinal epithelium guinea pig ClC-5 chloride channel.

Authors: I Cornejo, M I Niemeyer, F V Sepúlveda, L P Cid

Journal, date & volume: Biochim. Biophys. Acta, 2001 Jun 6 , 1512, 367-74

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/11406114

Abstract
We report the cloning of a guinea pig ClC-5 chloride channel (gpClC-5) from distal small intestinal epithelial cells by RT-PCR and RACE. The transcript is shown to be present in duodenum, jejunum and ileum epithelium by RT-PCR and Northern analysis. This is confirmed by in situ hybridisation which also shows the transcript to be homogeneously distributed in the crypt and villus regions. Expression of gpClC-5 in HEK-293 cells generated markedly outwardly rectified chloride currents with a perm-selectivity sequence of NO(3)(-)>Cl(-)>Br(-)>I(-)>F(-)>gluconate(-). The possible role of gpClC-5 in this epithelial location is discussed.