Referenced in: none

Automatically associated channels: Kir1.1 , Kir2.1 , Kir3.1

Title: Expression cloning of KCRF, a potassium channel regulatory factor.

Authors: T Keren-Raifman, T Ivanina, Y Bismuth, N Dascal

Journal, date & volume: Biochem. Biophys. Res. Commun., 2000 Aug 11 , 274, 852-8

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/10924366

Abstract
By functional coexpression screening of a rat cDNA library in Xenopus oocytes, we have cloned a protein (KCRF: K Channel Regulatory Factor) that reduces currents of several K(+) channels: G protein-activated GIRK1/4 (K(ir)3.1/K(ir)3.4), inward rectifier IRK1 (K(ir)2.1), and voltage-dependent K(V)1.1/K(V)beta1.1. KCRF did not modulate two other K(+) channels: ROMK1 (K(ir)1.1) and GIRK1/2 (K(ir)3.1/K(ir)3.2) and the voltage-dependent L-type Ca(2+) channels. Western blot analysis showed that KCRF is ubiquitous in rat tissues. Biochemical and electrophysiological experiments revealed that coexpression of KCRF causes a decrease in the level of expression of IRK1 and K(V)1.1/K(V)beta1.1 proteins in the oocytes.