Channelpedia

PubMed 11245596


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Kv1.3 , Kv1.5 , SK2 , SK3



Title: K+ channels and the microglial respiratory burst.

Authors: R Khanna, L Roy, X Zhu, L C Schlichter

Journal, date & volume: Am. J. Physiol., Cell Physiol., 2001 Apr , 280, C796-806

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/11245596


Abstract
Microglial activation following central nervous system damage or disease often culminates in a respiratory burst that is necessary for antimicrobial function, but, paradoxically, can damage bystander cells. We show that several K+ channels are expressed and play a role in the respiratory burst of cultured rat microglia. Three pharmacologically separable K+ currents had properties of Kv1.3 and the Ca2+/calmodulin-gated channels, SK2, SK3, and SK4. mRNA was detected for Kv1.3, Kv1.5, SK2, and/or SK3, and SK4. Protein was detected for Kv1.3, Kv1.5, and SK3 (selective SK2 and SK4 antibodies not available). No Kv1.5-like current was detected, and confocal immunofluorescence showed the protein to be subcellular, in contrast to the robust membrane localization of Kv1.3. To determine whether any of these channels play a role in microglial activation, a respiratory burst was stimulated with phorbol 12-myristate 13-acetate and measured using a single cell, fluorescence-based dihydrorhodamine 123 assay. The respiratory burst was markedly inhibited by blockers of SK2 (apamin) and SK4 channels (clotrimazole and charybdotoxin), and to a lesser extent, by the potent Kv1.3 blocker agitoxin-2.