Referenced in: none

Automatically associated channels: ClC2 , ClC4 , Slo1

Title: Single-channel analysis of a ClC-2-like chloride conductance in cultured rat cortical astrocytes.

Authors: M Nobile, M Pusch, C Rapisarda, S Ferroni

Journal, date & volume: FEBS Lett., 2000 Aug 11 , 479, 10-4

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/10940379

Abstract
The single-channel behavior of the hyperpolarization-activated, ClC-2-like inwardly rectifying Cl- current (IClh), induced by long-term dibutyryl-cyclic-AMP-treated cultured cortical rat astrocytes, was analyzed with the patch-clamp technique. In outside-out patches in symmetrical 144 mM Cl-solutions, openings of hyperpolarization-activated small-conductance Cl channels revealed burst activity of two equidistant conductance levels of 3 and 6 pS. The unitary openings displayed slow activation kinetics. The probabilities of the closed and conducting states were consistent with a double-barrelled structure of the channel protein. These results suggest that the astrocytic ClC-2-like Cl- current Iclh is mediated by a small-conductance Cl channel, which has the same structural motif as the Cl- channel prototype CIC-0.