Referenced in: none

Automatically associated channels: ClC1 , ClC2 , ClC3 , ClC4 , ClC7

Title: Mast cells express chloride channels of the ClC family.

Authors: M Kulka, A Schwingshackl, A D Befus

Journal, date & volume: Inflamm. Res., 2002 Sep , 51, 451-6

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/12365718

Abstract
The expression of specific Cl- channels in mast cells (MC) is poorly understood. Because the largest and most ubiquitously expressed family of Cl- channels is the ClC, we studied MC mRNA and protein expression for members of the ClC family.Specific primers were designed to ClC-1 to -7 and reverse-transcriptase polymerase chain reaction (RT-PCR) was performed on RNA from highly purified rat peritoneal mast cells (PMC) and the rat cultured mast cell line (RCMC). Protein expression of ClC-2, -3 and -7 chloride channels in PMC and RCMC was studied using western blotting.RT-PCR showed that RCMC expressed mRNA for the Cl- channels (ClC)-2, -3, -4, -5 and -7, while PMC expressed ClC-7. Using Western Blotting, we found protein expression of ClC-2 and ClC-7, but not ClC-3, in RCMC, whereas in PMC neither of these proteins could be detected.These results indicate that MC express several members of the ClC family and that these Cl- channels might be important in MC functions.