PubMed 12060804

Referenced in: none

Automatically associated channels: Kir1.1 , Kir2.1 , Kir2.2 , Kir2.3 , Kir2.4 , Kir3.1 , Kir3.3 , Kir3.4 , Kir4.1 , Kir4.2 , Kir5.1 , Kir6.1 , Kir6.2

Title: Diversity of Kir channel subunit mRNA expressed by retinal glial cells of the guinea-pig.

Authors: Maik Raap, Bernd Biedermann, Peter Braun, Ivan Milenkovic, Serguei N Skatchkov, Andreas Bringmann, Andreas Reichenbach

Journal, date & volume: Neuroreport, 2002 Jun 12 , 13, 1037-40

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/12060804

Abstract
One of the main functions of Müller glial cells is the performance of retinal K+ homeostasis which is thought to be primarily mediated by K+ fluxes through inwardly rectifying K+ (Kir) channels expressed in Müller cell membranes. Until now, there is limited knowledge about the types of Kir channel subunits expressed by Müller cells. Using RT-PCR, we investigated the expression of mRNA encoding different Kir channel subunits in the retina of the guinea pig. In order to verify expression by Müller cells, primary cultures of guinea pig Müller cells were also investigated. Both retinae and cultured Müller cells express mRNA for a diversity of Kir channel subtypes which include members of at least four channel subfamilies: Kir2.1, Kir2.2, Kir2.4, Kir3.1, Kir 3.2, Kir4.1, Kir6.1, and Kir6.2. mRNAs for the following Kir channel subtypes were not detected in Müller cells: Kir1.1, Kir2.3, Kir3.3, Kir3.4, Kir4.2, and Kir5.1. It is concluded that the spatial buffering of extracellular K+ by Müller cells may be mediated by cooperation of different subtypes of Kir channels, and that the distinct Kir channel types involved in this function may change depending on the physiological or metabolic state of the retina.