Channelpedia

PubMed 12860925


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: SK2



Title: Role of TASK2 potassium channels regarding volume regulation in primary cultures of mouse proximal tubules.

Authors: Herve Barriere, Radia Belfodil, Isabelle Rubera, Michel Tauc, Florian Lesage, Chantal Poujeol, Nicolas Guy, Jacques Barhanin, Philippe Poujeol

Journal, date & volume: J. Gen. Physiol., 2003 Aug , 122, 177-90

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/12860925


Abstract
Several papers reported the role of TASK2 channels in cell volume regulation and regulatory volume decrease (RVD). To check the possibility that the TASK2 channel modulates the RVD process in kidney, we performed primary cultures of proximal convoluted tubules (PCT) and distal convoluted tubules (DCT) from wild-type and TASK2 knockout (KO) mice. In KO mice, the TASK2 coding sequence was in part replaced by the lac-Z gene. This allows for the precise localization of TASK2 in kidney sections using beta-galactosidase staining. TASK2 was only localized in PCT cells. K+ currents were analyzed by the whole-cell clamp technique with 125 mM K-gluconate in the pipette and 140 mM Na-gluconate in the bath. In PCT cells from wild-type mice, hypotonicity induced swelling-activated K+ currents insensitive to 1 mM tetraethylammonium, 10 nM charybdotoxin, and 10 microM 293B, but blocked by 500 microM quinidine and 10 microM clofilium. These currents were increased in alkaline pH and decreased in acidic pH. In PCT cells from TASK2 KO, swelling-activated K+ currents were completely impaired. In conclusion, the TASK2 channel is expressed in kidney proximal cells and could be the swelling-activated K+ channel responsible for the cell volume regulation process during osmolyte absorptions in the proximal tubules.