Referenced in: none

Automatically associated channels: ClC2 , ClC3 , ClC4

Title: Fundamental role of ClC-3 in volume-sensitive Cl- channel function and cell volume regulation in AGS cells.

Authors: Nan Ge Jin, Jin Kyoung Kim, Dong Ki Yang, Soo Jin Cho, Jung Mogg Kim, Eun Ju Koh, Hyun Chae Jung, Insuk So, Ki Whan Kim

Journal, date & volume: Am. J. Physiol. Gastrointest. Liver Physiol., 2003 Nov , 285, G938-48

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/12842831

Abstract
Volume regulation is essential for cell function, but it is unknown which channels are involved in a regulatory volume decrease (RVD) in human gastric epithelial cells. Exposure to a hypotonic solution caused the increase in AGS cell volume, followed by the activation of a current. The reversal potential of the swelling-induced current suggested that Cl- was the primary charge carrier. The selectivity sequence for different anions was I- > Br- > Cl- > F- > gluconate. This current was inhibited by flufenamate, DIDS, tamoxifen, and 5-nitro-2-(3-phenylpropylamino)benzoate. Intracellular dialysis of three different anti-ClC-3 antibodies abolished or attenuated the Cl- current and disrupted RVD, whereas the current and RVD was unaltered by anti-ClC-2 antibody. Immunoblot studies demonstrated the presence of ClC-3 protein in Hela and AGS cells. RT-PCR analysis detected expression of ClC-3, MDR-1, and pICln mRNA in AGS cells. These results suggest a fundamental role of endogenous ClC-3 in the swelling-activated Cl- channels function and cell volume regulation in human gastric epithelial cells.