Referenced in: none

Automatically associated channels: Kv1.4 , Kv1.5 , Kv2.1 , Kv3.1 , Kv4.2 , Slo1

Title: [Effects of chronic exposure to beta-amyloid-peptide25-35 on the mRNA expressions of voltage-gated outward potassium channel subunits in cultured rat hippocampal neurons]

Authors: Hong-Wei Jin, Wei Zhang, Xiao-Liang Wang

Journal, date & volume: Yao Xue Xue Bao, 2002 Aug , 37, 598-602

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/12567771

Abstract
To investigate mRNA expression changes of voltage-gated outward potassium channel subtypes in cultured rat hippocampal neurons after chronic exposure to beta-amyloid-petitde25-35 (beta-AP25-35).mRNA expression was detected by RT-PCR, comparative expression levels were determined by imaging densitometer.Delayed rectifying (Kv2.1, Kv1.5), transient outward (Kv1.4, Kv4.2) and large conductance calcium-activated (rSlo) potassium channel mRNA were expressed in cultured rat hippocampal. In the presence of beta-AP25-35 3 mumol.L-1 for 24 h, the relative expression level of Kv2.1 was significantly increased (n = 3, P < 0.05); the other subtypes were not changed obviously (n = 3, P > 0.05). The increase of Kv2.1 mRNA mainly happened between 24 and 36 h after exposure to beta-AP25-35. After exposure to beta-AP25-35 for 60 h, Kv2.1 mRNA decreased significantly (n = 3, P < 0.01).The upregulation of Kv2.1 on transcription levels may be involved in the enhancement of delayed rectifying outward potassium (Ik) current induced by beta-AP25-35.