Referenced in: none

Automatically associated channels: Kir6.1 , Kir6.2

Title: Molecular basis of ATP-sensitive K+ channels in rat vascular smooth muscles.

Authors: Kun Cao, Guanghua Tang, Dahai Hu, Rui Wang

Journal, date & volume: Biochem. Biophys. Res. Commun., 2002 Aug 16 , 296, 463-9

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/12163042

Abstract
ATP-sensitive K+ (K(ATP)) channels couple metabolic changes to membrane excitability in vascular smooth muscle cells (SMCs). While the electrophysiological properties of K(ATP) channels have been examined, little is known about the molecular basis of K(ATP) complex in vascular SMCs. We identified and cloned four K(ATP) subunit genes from rat mesenteric artery, namely rvKir6.1, rvKir6.2, rvKirSUR1, and rvSUR2B. These clones showed over 99.6% amino acid sequence identity with other previously reported isoforms. The mRNA expression patterns of the K(ATP) subunits varied among rat aorta, mesenteric artery, pulmonary artery, tail artery, hepatic artery, and portal vein. Heterologous co-expression of rvKir6.1 and rvSUR2B yielded functional K(ATP) channels that were inhibited by glibenclamide, and opened by pinacidil. Our results for the first time reported the expression of four K(ATP) subunits in same vascular tissues, unmasking the diversity of native K(ATP) channels in vascular SMCs.