Channelpedia

PubMed 12133841


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Kv1.1



Title: Structure of the BgK-Kv1.1 complex based on distance restraints identified by double mutant cycles. Molecular basis for convergent evolution of Kv1 channel blockers.

Authors: Bernard Gilquin, Judith Racapé, Anja Wrisch, Violeta Visan, Alain Lecoq, Stephan Grissmer, André Ménez, Sylvaine Gasparini

Journal, date & volume: J. Biol. Chem., 2002 Oct 4 , 277, 37406-13

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/12133841


Abstract
A structural model of BgK, a sea anemone toxin, complexed with the S5-S6 region of Kv1.1, a voltage-gated potassium channel, was determined by flexible docking under distance restraints identified by a double mutant cycles approach. This structure provides the molecular basis for identifying the major determinants of the BgK-Kv1.1 channel interactions involving the BgK dyad residues Lys(25) and Tyr(26). These interactions are (i) electrostatic interactions between the extremity of Lys(25) side chain and carbonyl oxygen atoms of residues from the channel selectivity filter that may be strengthened by solvent exclusion provided by (ii) hydrophobic interactions involving BgK residues Tyr(26) and Phe(6) and Kv1.1 residue Tyr(379) whose side chain protrudes in the channel vestibule. In other Kv1 channel-BgK complexes, these interactions are likely to be conserved, implicating both conserved and variable residues from the channels. The data suggest that the conservation in sea anemone and scorpion potassium channel blockers of a functional dyad composed of a lysine, and a hydrophobic residue reflects their use of convergent binding solutions based on a crucial interplay between these important conserved interactions.