Referenced in: none

Automatically associated channels: Kv1.3 , Slo1

Title: Recombinant Kv1.3 potassium channels stabilize tonic firing of cultured rat hippocampal neurons.

Authors: Jürgen Kupper, Astrid A Prinz, Peter Fromherz

Journal, date & volume: Pflugers Arch., 2002 Feb , 443, 541-7

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/11907820

Abstract
We transfected cultured hippocampal neurons with the cDNA of the voltage-gated K+ channel Kv1.3 to investigate the mechanisms by which a specific ion channel influences excitability. In transfected neurons under voltage clamp we observed an additional outward current that was blocked selectively by margatoxin. Under current-clamp conditions, Kv1.3-expressing neurons fired tonically over a wide range of stimulation intensity. In non-transfected neurons, or in Kv1.3-expressing cells blocked with margatoxin, only a few action potentials were elicited before a stationary depolarized state was reached. We attribute the specific effect of Kv1.3 to its particularly slow deactivation near the resting potential. A computational model showed that a continuous outwards current arises in Kv1.3-expressing neurons during the interspike intervals. It expands the dynamic range so that these neurons still fire tonically at stimulus current intensities at which non-transfected cells have already been driven into a stationary depolarized state.