Referenced in: none

Automatically associated channels: Kv2.1 , Slo1

Title: Slowed inactivation at positive potentials in a rat axonal K+ channel is not due to preferential closed-state inactivation.

Authors: A Babes, E Lörinczi, V Ristoiu, M L Flonta, G Reid

Journal, date & volume: Physiol Res, 2001 , 50, 557-65

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/11829316

Abstract
We have investigated slow inactivation in a rat axonal K+ channel, the I channel. Using voltage steps to potentials between -70 mV and +80 mV, from a holding potential of -100 mV, we observed a marked slowing of inactivation at positive potentials: the time constant was 4.5+/-0.4 s at -40 mV (mean +/- S.E.M.), increasing to 14.7+/-2.0 s at +40 mV. Slowed inactivation at positive potentials is not consistent with published descriptions of C-type inactivation, but can be explained by models in which inactivation is preferentially from closed states (which have been developed for Kv2.1 and some Ca2+ channels). We tested two predictions of preferential closed-state models: inactivation should be more rapid during a train of brief pulses than during a long pulse to the same potential, and the cumulative inactivation measured with paired pulses should be greater than the inactivation at the same time during a continuous pulse. The I channel does not behave according to these predictions, indicating that preferential closed-state inactivation does not explain the slowing of inactivation we observe at positive potentials. Inactivation of the I channel therefore differs both from C-type inactivation, as presently understood, and from the inactivation of Kv2.1.