Referenced in: none

Automatically associated channels: ClC2 , ClC4

Title: ClC-2 in guinea pig colon: mRNA, immunolabeling, and functional evidence for surface epithelium localization.

Authors: Marcelo Catalán, Isabel Cornejo, Carlos D Figueroa, María Isabel Niemeyer, Francisco V Sepúlveda, L Pablo Cid

Journal, date & volume: Am. J. Physiol. Gastrointest. Liver Physiol., 2002 Oct , 283, G1004-13

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/12223361

Abstract
The principal function of the colon in fluid homeostasis is the absorption of NaCl and water. Apical membrane Na(+) channels, Na(+)/H(+) and Cl(-)/HCO exchangers, have all been postulated to mediate NaCl entry into colonocytes. The identity of the basolateral exit pathway for Cl(-) is unknown. We have previously demonstrated the presence of the ClC-2 transcript in the guinea pig intestine. Now we explore in more detail, the tissue and cellular distribution of chloride channel ClC-2 in the distal colon by in situ hybridization and immunohistochemistry. The patch-clamp technique was used to characterize Cl(-) currents in isolated surface epithelial cells from guinea pig distal colon and these were compared with those mediated by recombinant guinea pig (gp)ClC-2. ClC-2 mRNA and protein were found in the surface epithelium of the distal colon. Immunolocalization revealed that, in addition to some intracellular labeling, ClC-2 was present in the basolateral membranes but absent from the apical pole of colonocytes. Isolated surface epithelial cells exhibited hyperpolarization-activated chloride currents showing a Cl(-) > I(-) permeability and Cd(2+) sensitivity. These characteristics, as well as some details of the kinetics of activation and deactivation, were very similar to those of recombinant gpClC-2 measured in parallel experiments. The presence of active ClC-2 type currents in surface colonic epithelium, coupled to a basolateral location for ClC-2 in the distal colon, suggests a role for ClC-2 channel in mediating basolateral membrane exit of Cl(-) as an essential step in a NaCl absorption process.