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PubMed 15130477


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: SK2



Title: Crystal structures of apocalmodulin and an apocalmodulin/SK potassium channel gating domain complex.

Authors: Maria A Schumacher, Matthew Crum, Marshall C Miller

Journal, date & volume: Structure, 2004 May , 12, 849-60

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/15130477


Abstract
Small conductance Ca2+-activated K+ channels (SK channels) are composed of the pore-forming alpha subunit and calmodulin (CaM). CaM binds to a region of the alpha subunit called the CaM binding domain (CaMBD), located intracellular and immediately C-terminal to the inner helix gate, in either the presence or absence of Ca2+. SK gating occurs when Ca2+ binds the N lobe of CaM thereby transmitting the signal to the attached inner helix gate to open. Here we present crystal structures of apoCaM and apoCaM/SK2 CaMBD complex. Several apoCaM crystal forms with multiple (12) packing environments reveal the same EF hand domain-swapped dimer providing potentially new insight into CaM regulation. The apoCaM/SK2 CaMBD structure, combined with our Ca2+/CaM/CaMBD structure suggests that Ca2+ binding induces folding and dimerization of the CaMBD, which causes large CaMBD-CaM C lobe conformational changes, including a >90 degrees rotation of the region of the CaMBD directly connected to the gate.